Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

Abstract Clarias gariepinus growth hormone ( Cg GH) transgenesis was previously used to develop a population of second-generation (G 2 ) transgenic mutiara catfish C. ). The third generation these fish (1-month old fingerlings) had 2–3 times improvement in compared non-transgenic the commercial feed test for 6 weeks rearing. We assessed impact GH transgene expression on and protein amino acid content G 3 relative catfish. Since variation composition mixes can affect fish, we tested three mixtures boiled tuna Euthynnus affinis ): A (50:50 tuna), B (65:35), C (80:20) Feed A* (50:50) fed as control efficiency, including conversion ratio use efficiency (i.e., retention productive value), assessed. content, essential (feed B) were higher than A*). latter deficient lysine methionine. Transgenic with showed deficiency lower feeds B. (65:35) optimal mixture utilized; it increased growth, levels, when supported by feeding balanced nutrients.